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Title

 

University ID

5997

ShowOnHomePage

Yes

Full Name

 

First Name

Joseph

Last Name

Jez

Degree

 

Faculty Keyword

biochemistry, kinetics, plant biology, protein engineering, protein structure, structural biology

Office Phone

314-935-3376

Lab Phone

314-935-4085

Other Phone

 

Fax

 

Lab Address

313 Rebstock Hall

Email

 

Research Abstract

Plants are amazing chemists capable of generating an array of small molecules with different biological effects. The goal of our research is to understand the molecular machinery involved in assembling these compounds. Currently, we are studying the biosynthesis of peptides that protect plants from heavy metal toxicity and environmental stresses using approaches that blend biochemistry, plant biology, and x-ray crystallography. Understanding how plants synthesize these peptides provides a foundation for engineering their production. For example, in Arabidopsis and other plants, phytochelatin synthase (PCS) catalyzes the formation of heavy metal chelating peptides. Recently, we re-engineered PCS using directed evolution methods to improve tolerance to cadmium and are evaluating the effect of expressing the engineered PCS on cadmium tolerance and accumulation in plants. Future work will explore the potential of this engineered system as a bioremediation tool. In a related project, we are examining glutathione biosynthesis in plants. Although glutathione is a key component in a plant’s stress response network, the structural and functional details of the proteins that synthesize it, as well as the potential biochemical mechanisms of their regulation, remain unexplored. Our initial investigations suggest that cellular redox environment regulates a key step in this pathway through a thiol-based switching mechanism. This work may lead to more general models of how redox environment regulates protein function in plants. We are also collaborating with Divergence, Inc. (St. Louis, MO) to mechanistically and structurally characterize new protein targets for the development of compounds targeting parasitic nematodes.

Middle Initial

M.

Public Name

Joseph M. Jez

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Doctoral Degree2

 

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Division Primary Department

 

Campus Box

1137

Website Url

http://biology4.wustl.edu/faculty/jez

Ten Word Res Desc

Structural biology and biochemistry of plants

Micro Category1

 

Micro Category2

 

Micro Category3

 

Plant Category

 

Comp Category

 

DBBS Faculty

 

Archived Faculy Flag

 

Date Last Updated

11/10/2011 11:35 AM

Publications

Lee SG, Haakenson W, McCarter JP, Williams DJ, Hresko MC, Jez JM (2011) Thermodynamic evaluation of ligand binding in the plant-like phosphoethanolamine methyltransferases of the parasitic nematode Haemonchus contortus. J. Biol. Chem. 2011 286: 38060-38068.
 
Alvarez S, Galant A, Jez JM, Hicks LM.  Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics. Proteomics 2011 11: 1346-1135.
 
Chen Q, Westfall CS, Hicks LM, Wang S, Jez JM.  Kinetic basis for the conjugation of auxin by a GH3 family indole acetic acid-amido synthetase. J. Biol. Chem. 2010 285: 29780-29786.
 
Schroeder AC, Zhu C, Yanamadala SR, Cahoon RE, Arkus KAJ, Wachsstock L, Bleeke J, Krishnan HB, Jez JM. Threonine-insensitive homoserine dehydrogenase from soybean: genomic organization, kinetic characterization, and in vivo activity. J. Biol. Chem.
2010 Jan 8;285(2):827-34. Epub 2009 Nov 6. 
Galant A, Arkus KAJ, Zubieta C, Cahoon RE, Jez JM. Structural basis for evolution of product diversity in soybean glutathione synthesis. Plant Cell 2009 21: 3450-3458.

Show Bio Page

Yes

Exclude Email

No

Thumbnail_Image_Url

http://dbbs.wustl.edu/Faculty Photos Thumbnail/Jez_J.jpg

Profile_Image_Url

http://dbbs.wustl.edu/Faculty Photos/Jez_J.jpg

Primary Program

 

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Approval Status Approved
 

Attachments

Created at 11/10/2011 11:35 AM by DBBS_SP_SAPP
Last modified at 11/10/2011 12:40 PM by Kathryn Ruzicka