Jesus Acevedo Cintron (MSTP in PhD training)

  • Carolina, Puerto Rico

  • University of Puerto Rico-Rio Piedras (2015)

  • Developmental, Regenerative and Stem Cell Biology

  • Susan Mackinnon, MD

  • The role of the inflammatory microenvironment in Acellular Nerve Allografts (ANAs)

  • jacevedo@wustl.edu

Research

Reconstruction of nerve gap injuries continue to pose a challenge. While nerve autografts are still the gold standard to repair nerve gaps, alternatives, including acellular nerve allografts (ANAs), are highly desired. However, the regenerative potential of these alternatives is restricted by their length, with long ANAs (≥4 cm) supporting limited axonal regeneration. Using a sciatic nerve injury model, we are interested in studying and characterizing differences in the formation and morphology of blood vessels formed inside the ANA, the accumulation of immune cells and the expression of cytokines between short (2 cm) and long (4 cm) ANAs. Preliminary data using morphometric histology shows robust nerve regeneration in 2 cm but not 4 cm ANAs, changes in blood vessel morphology and an increase in the infiltration of immune cells in the 4 cm ANAs when compared to the 2 cm ANAs. This suggests an on-going inflammatory process is happening in the 4 cm ANAs and that this process is causing changes in the morphology of the blood vessels and potentially blocking nerve regeneration in long ANAs. Future experiments will focus on identifying the inflammatory and immune cells present inside the long ANAs, and on using drugs to decrease inflammation inside these long ANAs.

Graduate Publications:

Pan D, Schellhardt L, Acevedo-Cintron JA, Hunter D, Snyder-Warwick AK, Mackinnon SE, Wood MD. 2022 IL-4 expressing cells are recruited to nerve after injury and promote regeneration. Exp Neurol, 347():113909.

Sayanagi J, Acevedo-Cintrón JA, Pan D, Schellhardt L, Hunter DA, Snyder-Warwick AK, Mackinnon SE, Wood MD. 2021 Brief Electrical Stimulation Accelerates Axon Regeneration and Promotes Recovery Following Nerve Transection and Repair in Mice. J Bone Joint Surg Am, 103(20):e80.

Pan D, Sayanagi J, Acevedo-Cintrón JA, Schellhardt L, Snyder-Warwick AK, Mackinnon SE, Wood MD. 2021 Liposomes embedded within fibrin gels facilitate localized macrophage manipulations within nerve. J Neurosci Methods, 348():108981.

Last Updated: 9/17/2019 1:15:02 PM

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