Jacco Boon, Ph.D.

Associate Professor
Internal Medicine
Infectious Diseases
Molecular Microbiology
Pathology and Immunology

Molecular Microbiology and Microbial Pathogenesis Program
Immunology Program

  • 314-286-0857

  • 314-286-1522

  • 314-362-9230

  • 7240 McDonnell Pediatric Research Building

  • jboon@wustl.edu

  • host-pathogen interactions, Influenza virus, Viral pathogenesis, Bourbon virus, RNA biology, Influenza Reassortment

  • Pathogenesis of Influenza A viruses

Research Abstract:

Influenza A virus is a collective name for a large number of different viruses that can infect many different avian and mammalian species. Humans are susceptible to a small subset of influenza A viruses; seasonal H1N1 and H3N2 viruses cause yearly epidemics and are associated with mild disease in healthy adults, while highly pathogenic influenza A viruses, like H5N1, induce severe disease and mortality in 60% of the reported cases. Pathogenesis, or the ability to cause severe disease, is the result of a complex interplay between the virus and the infected host. We are interested in identifying novel host factors and mechanisms that are involved in influenza pathogenesis. To do this, we generate novel gene-knockout mice and evaluate their disease after influenza virus or vesicular stomatitis virus infection. We also make use of natural genetic variation between different mouse strains to discover novel host factors that mediate influenza disease severity and antibody responses after influenza vaccination.

Another program in the lab is centered around the question of how the segmented genome of influenza virus is packaged. Furthermore, we want to understand how this process impacts virus reassortment and thus the generation of pandemic influenza viruses. We recently adapted a state-of-the-art technology, called PAR-CLIP, to influenza A virus and identified novel structures and features in the viral genome that are required for genome packaging and replication. Our future goals are to determine (i) how conserved these RNA structures are among human, avian and swine isolates of influenza A virus, (ii) how they function in the context of genome packaging, (iii) how they modulate virus reassortment.

Finally, we are interested in emerging tick-borne viruses, such as Bourbon virus. We have recently generated a molecular clone of the virus and developed an animal model to study cell and tissue tropism as well as evaluate potential anti-viral therapies against this emerging deadly disease.

Selected Publications:

Gounder AP, Yokoyama CC, Jarjour NN, Bricker TL, Edelson BT, and Boon ACM (2018). Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer. PLoS Pathogens; 14(4):e1007001.

Williams GD, Townsend D, Wylie C, Amarasinghe GK, Kutluay SB, Boon ACM (2018) Nucleotide resolution mapping of influenza A virus nucleoprotein-RNA interactions reveals the landscape of viral RNA features required for replication. Nat Communications; 9(1):465.

Nair S, Huynh JP, Lampropoulou V, Loginicheva E, Esaulova E, Gounder AP, Boon ACM, Schwarzkopf EA, Bradstreet TR, Edelson BT, Artyomov MN, Stallings CL, Diamond MS (2018). Irg1 expression in myeloid cells prevents immunopathology during M. tuberculosis infection. Journal of Experimental Medicine; 215(4):1035-1045.

Steed AL, Christophi GP, Kaiko GE, Sun L, Goodwin VM, Jain U, Esaulova E, Artyomov MN, Morales DJ, Holtzman MJ, Boon ACM, Lenschow DJ, Stappenbeck TS(2017). The microbial metabolite desaminotyrosine protects from influenza through type I interferon. Science (4); 357(6350):498-502.

DesRochers BL, Chen RE, Gounder AP, Pinto AK, Bricker T, Linton CN, Rogers CD, Williams GD, Webby RJ, Boon ACM. (2016). Residues in the PB2 and PA genes contribute to the pathogenicity of avian H7N3 influenza A virus in DBA/2 mice. Virology. Jul;494:89-99.

Williams GD, Pinto AK, Doll B, Boon ACM. (2016). A North American H7N3 Influenza Virus Supports Reassortment with 2009 Pandemic H1N1 and Induces Disease in Mice without Prior Adaptation. Journal of Virology. 14;90(9):4796-806.

Lu Q, Yokoyama CC, Williams JW, Baldridge MT, Jin X, DesRochers B, Bricker T, Wilen CB, Bagaitkar J, Loginicheva E, Sergushichev A, Kreamalmeyer D, Keller BC, Zhao Y, Kambal A, Green DR, Martinez J, Dinauer MC, Holtzman MJ, Crouch EC, Beatty W, Boon ACM, Zhang H, Randolph GJ, Artyomov MN, Virgin HW. (2016). Homeostatic Control of Innate Lung Inflammation by Vici Syndrome Gene Epg5 and Additional Autophagy Genes Promotes Influenza Pathogenesis. Cell Host and Microbe. 13;19(1):102-13.

Pinto AK, Williams GD, Szretter KJ, White JP, Proença-Módena JL, Liu G, Olejnik J, Brien JD, Ebihara H, Mühlberger E, Amarasinghe G, Diamond MS, Boon ACM. (2015). Human and Murine IFIT1 Proteins Do Not Restrict Infection of Negative-Sense RNA Viruses of the Orthomyxoviridae, Bunyaviridae, and Filoviridae Families. Journal of Virology, 89(18):9465-76.

Last Updated: 7/31/2018 2:10:26 PM

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