Dmitri A. Nusinow, PhD

Assistant Member
Donald Danforth Plant Science Center
Honorary Adjunct Assistant Professor

Plant and Microbial Biosciences Program
Molecular Cell Biology Program
Biochemistry, Biophysics, and Structural Biology Program

  • 314-587-1489

  • 314-587-1589

  • 975 N. Warson Road, St Louis, MO 63132



  • Plant Biology, circadian rhythms, biochemistry, genetics, Arabidopsis, mass spectrometry, chronobiology

  • Circadian clock regulation of physiology and development in plants.

Research Abstract:

Time measurement is critical in biology. Light and temperature oscillate each day with a period of approximately 24 hours. Selective pressure has led to the repeated evolution of an environmentally responsive, endogenous timekeeper that permits for the anticipation and measurement of these daily cycles. The circadian clock allows for the resonance of internal and environmental oscillations, providing an adaptive advantage through the coordination of physiology and development with daily and seasonal change. While humans monitor their watches to know when to eat, meet, and sleep, plants use their internal clock to anticipate sunrise to prepare to harvest photons, measure day-length for tracking seasonal change and to meter out resources to ensure that energy reserves last throughout the night.

The Nusinow lab uses a combination of molecular, biochemical, genetic, genomic, and proteomic tools to uncover the molecular connections between signaling networks, the circadian oscillator, and specific outputs (e.g. growth, photosynthesis, flowering, starch accumulation, and defense) in plants and microbes.

Selected Publications:

Huang, H., Yoo, C. Y., Bindbeutel, R., Goldsworthy, J., Tielking, A., Alvarez, S., Naldrett, M. J., Evans, B. S., Chen, M., and Nusinow, D. “PCH1 Integrates Circadian and Light-Signaling Pathways to Control Photoperiod-Responsive Growth in Arabidopsis.” eLife 5, (2016): doi:10.7554/eLife.13292

Huang, H., Alvarez, S., Bindbeutel, R., Shen, Z., Naldrett, M. J., Evans, B. S., Briggs, S. P., Hicks, L. M., Kay, S. A., and Nusinow, D. “Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry.” Molecular & cellular proteomics : MCP 15, no. 1 (2016): 201–217. doi:10.1074/mcp.M115.054064

Kaiserli, E., Páldi, K., O`Donnell, L., Batalov, O., Pedmale, U. V., Nusinow, D., Kay, S. A., and Chory, J. “Integration of Light and Photoperiodic Signaling in Transcriptional Nuclear Foci.” Developmental cell 35, no. 3 (2015): 311–321. doi:10.1016/j.devcel.2015.10.008

Waadt, R., Manalansan, B., Rauniyar, N., Munemasa, S., Booker, M. A., Brandt, B., Waadt, C., Nusinow, D., Kay, S. A., Kunz, H.-H., Schumacher, K., DeLong, A., Yates, J. R., and Schroeder, J. I. “Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-Fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses.” PLANT PHYSIOLOGY 169, no. 1 (2015): 760–779. doi:10.1104/pp.15.00575

Chow, B. Y., Helfer, A., Nusinow, D., and Kay, S. A. “ELF3 Recruitment to the PRR9 Promoter Requires Other Evening Complex Members in the Arabidopsis Circadian Clock.” Plant signaling & behavior 7, no. 2 (2012): 170–173. doi:10.4161/psb.18766

Nusinow, D., Helfer, A., Hamilton, E. E., King, J. J., Imaizumi, T., Schultz, T. F., Farré, E. M., and Kay, S. A. “The ELF4-ELF3-LUX Complex Links the Circadian Clock to Diurnal Control of Hypocotyl Growth.” Nature 475, no. 7356 (2011): 398–402. doi:10.1038/nature10182

Helfer, A., Nusinow, D., Chow, B. Y., Gehrke, A. R., Bulyk, M. L., and Kay, S. A. “LUX ARRHYTHMO Encodes a Nighttime Repressor of Circadian Gene Expression in the Arabidopsis Core Clock” Current Biology 21, no. 2 (2011): 126–133. doi:10.1016/j.cub.2010.12.021

Zhang, E. E., Liu, Y., Dentin, R., Pongsawakul, P. Y., Liu, A. C., Hirota, T., Nusinow, D., Sun, X., Landais, S., Kodama, Y., Brenner, D. A., Montminy, M., and Kay, S. A. “Cryptochrome Mediates Circadian Regulation of cAMP Signaling and Hepatic Gluconeogenesis.” Nature medicine 16, no. 10 (2010): 1152–1156. doi:10.1038/nm.2214

Sawa, M., Nusinow, D., Kay, S. A., and Imaizumi, T. “FKF1 and GIGANTEA Complex Formation Is Required for Day-Length Measurement in Arabidopsis.” Science (New York, NY) 318, no. 5848 (2007): 261–265. doi:10.1126/science.1146994

Nusinow, D., Hernández-Muñoz, I., Fazzio, T. G., Shah, G. M., Kraus, W. L., and Panning, B. “Poly(ADP-Ribose) Polymerase 1 Is Inhibited by a Histone H2A Variant, MacroH2A, and Contributes to Silencing of the Inactive X Chromosome.” The Journal of biological chemistry 282, no. 17 (2007): 12851–12859. doi:10.1074/jbc.M610502200

Last Updated: 3/18/2016 9:17:14 AM

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