R. Keith Slotkin, PhD

Full Member and Principal Investigator
Donald Danforth Plant Science Center
Honorary Adjunct Professor

Plant and Microbial Biosciences Program
Molecular Genetics and Genomics Program

  • Danforth Plant Science Center, St. Louis, MO

  • kslotkin@danforthcenter.org

  • https://slotkinlab.github.io/

  • https://twitter.com/Slotkin_Lab

  • transposable element, epigenetics, DNA methylation, chromatin, transgene silencing

  • Investigating how plant cells determine which fragments of DNA should be expressed (like genes), and which should be targeted for repression and not allowed to express

Research Abstract:

The Slotkin laboratory investigates how plant cells determine which fragments of DNA should be expressed (like genes), and which should be targeted for repression and not allowed to express. The regions of the genome that are targeted for repression are called Transposable Elements (TEs), which are mobile ‘jumping genes’ that cause mutations when they jump. Most of the time these new mutations generated by TEs hurt the cell or are inconsequential, but occasionally something new is produced that is helpful and will be selected for, leading to some spectacular examples of TE-induced gene or trait regulation.

In a broad sense, what the Slotkin laboratory aims to determine is how self-perpetuating feed-forward cycles in the cell are initiated. The cycles we work on are RNA interference (RNAi), RNA-directed DNA Methylation (RdDM) and maintenance epigenetic silencing. We have learned that these cycles can feed into each other. Our goal now is to determine how the first cycle is triggered to initiate.

Projects in the lab vary from proteomics to genomics, bench-work to purely computational, and basic fundamental questions to applied crop pest resistance. The majority of our research is done on the powerful model plant Arabidopsis, while through collaborations we have also concentrated on maize, soybeans and aphids, and bioinformatically on any plant genome. The unifying features of all of our projects are based on our in-lab expertise on: Transposable Elements, Plant Epigenetics, RNAi, RdDM, small RNAs, DNA methylation and the Bioinformatics of multi-copy regions of the genome.

Selected Publications:

See a full updated list at: https://scholar.google.com/citations?user=jkJlkSYAAAAJ&hl=en

Review Articles:

P. Liu, D. Cuerda-Gil, S. Shahid and R.K. Slotkin. (2022) The Epigenetic Control of the Transposable Element Lifecycle in Plant Genomes. Annual Review of Genetics

Y.-H. Hung and R.K. Slotkin. (2021) The initiation of RNA interference (RNAi) in plants. Current Opinion in Plant Biology v61:102014.

S. Shahid and R.K. Slotkin. (2020) The current revolution in transposable element biology enabled by long reads. Current Opinion in Plant Biology v54: 49-56.

R.K. Slotkin. (2018) The case for not masking away repetitive DNA. Mobile DNA v9: 475.

D. Cuerda-Gil and R.K. Slotkin. (2016) Non-Canonical RNA-directed DNA Methylation. Nature Plants v2: 16163.

M.J. Sigman and R.K. Slotkin. (2016) The First Rule of Plant Transposable Element Silencing: Location, Location, Location. The Plant Cell v28: 304-313.

D. Fultz, S.G. Choudury, and R.K. Slotkin. (2015) Silencing of Active Transposable Elements in Plants. Current Opinion in Plant Biology v27: 67-76

G. Martinez and R.K. Slotkin. (2012) Developmental Reactivation of Transposable Element Silencing in Plants: Functional or Byproduct? Current Opinion in Plant Biology v15: 496-502. http://www.sciencedirect.com/science/article/pii/S1369526612001136

A.D. McCue and R.K. Slotkin. (2012) Transposable Element Small RNAs as Regulators of Gene Expression. Trends in Genetics v28: 616-623.

A.D. McCue, M. Cresti, J.A. Feijo and R.K. Slotkin. (2011) Cytoplasmic connection of sperm cells to the pollen vegetative nucleus: the functional role of the male germ unit revisited. Journal of Experimental Botany v62: 1621-1631.

R.K. Slotkin. (2010) The Epigenetic Control of the Athila Family of Retrotransposons in Arabidopsis. Epigenetics v5: 483-490.

R.K. Slotkin and R.A. Martienssen. (2007) Transposable Elements and the Epigenetic Regulation of the Genome. Nature Reviews Genetics v8: 272-285.

Last Updated: 6/27/2022 8:21:43 PM

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